Journal: Cancers

Article Title: The 2EF Antibody Targets a Unique N-Terminal Epitope of Trop-2 and Enhances the In Vivo Activity of the Cancer-Selective 2G10 Antibody

doi: 10.3390/cancers15143721

Figure Lengend Snippet: Hu2EF immune-mediated killing of Trop-2-expressing cancer cells. ( A ) Flow cytometry analysis of Hu2EF binding to target MCF-7 cells. ( B ) Hu2EF-mediated ADCC dose-response curve, following incubation of MCF-7 target cells with serial dilutions of the Hu2EF mAb and effector Jurkat NF-kB/NFAT-reporter cells. Luminescence data upon NF-kB/NFAT-reporter activation (three replica wells per data point) were plotted against mAb concentration. Error bars: SEM.

Article Snippet: Jurkat NF-κB/NFAT (ADCC Reporter Bioassay, Core Kit, Cat. No. G7010, Promega, Madison, WI, USA) were utilized as effector/reporter cells.

Techniques: Expressing, Flow Cytometry, Binding Assay, Incubation, Activation Assay, Concentration Assay

Journal: PLoS ONE

Article Title: Structurally simplified biphenyl combretastatin A4 derivatives retain in vitro anti-cancer activity dependent on mitotic arrest

doi: 10.1371/journal.pone.0171806

Figure Lengend Snippet: ( A) Wild-type E6-1 leukemia cells and dominant-negative Fas-associated via death domain (DN FADD) leukemia cells were treated with the indicated drug at the indicated dose for 48 hours and the WST-1 reagent was used to quantify proliferation. Absorbance was read at 450 nm and expressed as a % of DMSO control. (B, C) Cells were treated for either (B) 24 hours or (C) 48 hours and were probed with annexin V and propidium iodide (PI). Percentage of annexin V and PI positive cells was quantified using image-based cytometry. Blue bar represents viable cells; orange bar represents PI and annexin V positive cells; green bar represents annexin V positive cells; red bar represents PI positive cells. Values are expressed as mean ± SD from three independent experiments. * p < 0.05 vs. DMSO control; **** p < 0.0001 vs. DMSO control.

Article Snippet: E6-1 jurkat cells (ATCC, Cat. No. TIB-152, Manassas, VA, USA), an acute T-cell leukemia cell line, as well as a jurkat cell line dominant negative for the Fas-Associated Death Domain (FADD) protein (DN-FADD Jurkat; ATCC, Cat. No. CRL-2572, Manassas, VA, USA), were cultured with RPMI-1640 (Sigma-Aldrich Canada, Mississauga, ON, Canada) supplemented with 10% (v/v) FBS standard (Thermo Scientific, Waltham, MA, USA).

Techniques: Dominant Negative Mutation, Control, Cytometry